Researchers at the National Institute of Plant Genome Research (NIPGR) have utilized CRISPR-Cas9 gene-editing to improve phosphate absorption in japonica rice, resulting in a 20–40% boost in yield with regulated phosphate application
What is Japonica Rice?
Japonica rice is a high-starch, short-grain type of rice mainly cultivated in East Asia, commonly utilized in breeding studies because of its simple genetic modification and consistent traits in regulated settings.
Created By:
Created by NIPGR, New Delhi, utilizing CRISPR-Cas9 gene modification.
Study released in the Plant Biotechnology Journal
Cultivar utilized: Nipponbare (a representative japonica type)
How Was It Created?
Target Gene Identified: OsPHO1;2, a phosphate transporter transferring phosphorus from roots to shoots.
Repressor Identified: OsWRKY6, an inhibitor of this transporter
First Strategy Unsuccessful: Disabling the repressor affected other functions in the plant.
Exact Editing: Only the 30 base-pair binding sites of the repressor were eliminated utilizing CRISPR.
Result: Enhanced phosphate transport to the shoot, resulting in improved seed formation.
Essential Characteristics of Gene-Modified Japonica Rice:
20% increased output with the complete fertilizer amount and a 40% yield boost with just 10% of the suggested phosphate.
Enhanced phosphate uptake resulting from better transporter stimulation.
Increased panicles and seeds, with seed quality and size staying consistent.
No foreign DNA in the final generation; eliminated through Mendelian segregation.
No unintended effects observed; confirmed with leading genome-prediction tools.
Effectively replicates minimally invasive promoter gene surgery.
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